Journal: Cancer Management and Research

Article Title: Long Non-Coding RNA TPTEP1 Exerts Tumor Suppressive Functions via Sequestering miR-4295 to Regulate Growth Arrest and DNA Damage-Inducible 45α Expression in Acute Myeloid Leukemia

doi: 10.2147/CMAR.S486875

Figure Lengend Snippet: TPTEP1 was downregulated in AML. ( A ) According to the GEPIA database, the level of TPTEP1 expression was various in multiple cancers. ( B ) The low expression patterns of TPTEP1 in AML samples were obtained from GEPIA. ( C ) Kaplan-Meier curve for overall survival in the cohort of 106 AML patients were obtained from GEPIA. ( D and E ) qRT-PCR was conducted to evaluate the relative expression of TPTEP1 in 35 AML patients and 12 normal individuals ( D ), AML cell lines and CD34 + cells ( E ). * P < 0.05, **P < 0.01.

Article Snippet: The CD34 + hematopoietic stem and progenitor cell (CD34 + ) were kindly provided Dr. Qingsheng Li (First Affiliated Hospital, Anhui Medical University, Hefei, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: Cancer Management and Research

Article Title: Long Non-Coding RNA TPTEP1 Exerts Tumor Suppressive Functions via Sequestering miR-4295 to Regulate Growth Arrest and DNA Damage-Inducible 45α Expression in Acute Myeloid Leukemia

doi: 10.2147/CMAR.S486875

Figure Lengend Snippet: Knockdown of miR-4295 inhibited AML cells growth. ( A, C ) qRT-PCR was performed to detect the expression of miR-4295 in 30 AML patient samples and 21 healthy controls ( A ), in CD34 + cells and AML cells ( C ). ( B ) Linear regression analysis between TPTEP1 and miR-4295 expression levels in AML samples. ( D–G ) AML cells were transfected with inhibitor or NC. The abundance of miR-4295 ( D ), proliferation ( E ), apoptosis ( F ), and colony formation ability ( G ) were evaluated in AML cells by qRT-PCR, CCK-8 assay, Annexin V/PI assay or soft agar colony formation assay, respectively. **P < 0.01.

Article Snippet: The CD34 + hematopoietic stem and progenitor cell (CD34 + ) were kindly provided Dr. Qingsheng Li (First Affiliated Hospital, Anhui Medical University, Hefei, China).

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Soft Agar Assay

Journal: Nature Communications

Article Title: miR-142 deficit in T cells during blast crisis promotes chronic myeloid leukemia immune escape

doi: 10.1038/s41467-025-56383-y

Figure Lengend Snippet: a – f Experimental design and results. A cohort of BC CML mice were treated with NIL (30 mg/kg/day, oral gavage), NIL+M-miR-142(30 mg/kg/day, iv), NIL+PD-1 Ab (10 mg/kg, 3x/week), NIL+M-miR-142+PD-1 Ab, or vehicle for 3 weeks ( a ; n = 15 per group). WBC counts ( b ), blood smear ( c ), leukemic cell engraftment and host T cell percentages in PB ( d ), and survival of the treated mice ( e ) are shown. BM cells from the treated mice were transplanted into 2nd recipients ( n = 15 for vehicle and NIL groups and n = 10 for the remaining three groups). Leukemic cell engraftment in PB and survival of the 2nd recipients ( f ) are shown. g – j Experimental design and results. A cohort of BC CML PDX mice were given autologous human T cells (10 6 /mouse on day 14) and 3 weeks’ treatment with vehicle, NIL (30 mg/kg/day, oral gavage), NIL+M-miR-142(30 mg/kg/day, iv), NIL+PD-1 Ab (10 mg/kg, 3x/week), or NIL+M-miR-142+PD-1 Ab ( g ). Blood smear ( h ), human (h) leukemic cell engraftment in PB ( i , left; % of hCD45 + minus % of hCD3 + ; n = 8 for vehicle and NIL groups; n = 9 for NIL+M-miR-142 and NIL+PD-1 Ab groups; n = 10 for NIL+M-miR-142+PD-1 Ab group) and survival ( i , right; n = 11 for vehicle group; n = 13 for NIL group; n = 14 for NIL+M-miR-142 and NIL+PD-1 Ab groups; n = 15 for NIL + M-miR-142 + PD-1 Ab group) were monitored. BM cells from the treated mice were transplanted into 2nd NSGS recipient mice ( n = 10 per group). Human cell engraftment in PB and survival of the 2nd recipients are shown ( j ). TKI tyrosine kinase inhibitor, Ab antibody, BC blast crisis, CML chronic myeloid leukemia, NIL nilotinib, WBC white blood cell, PB peripheral blood, 2nd: secondary; PDX: patient-derived xenograft. For b , d , f , i and j , comparisons among multi-groups were performed by one-way ANOVA. For e , f , i and j , log-rank test was used to compare survival curves among multi-groups. P values were corrected for multiple comparisons using Holm–Šídák method. Results shown represent mean ± SEM. For a and g , mouse images were created in BioRender. Chen, F. (2025) https://BioRender.com/e61c469 . Source data are provided as a Source Data file.

Article Snippet: To study the effect of M-miR-142 on human T cells, we generated BC CML PDX model by transplanting human BC CML CD34 + cells into NSGS (2Gy, The Jackson Laboratory) mice.

Techniques: Derivative Assay